To summarize "Sampling protocol for population genetics"

mardi 2 juin 2015
par  Dorian GUILLEMAIN, Romain DAVID
popularité : 9%

Index of "Sampling protocol for population genetics?"
1. Principle
2. Method
3. Sampling
4. Deliverables
5. To summarize
Back to protocols Index
How to cite this content :

David R., Arvanitidis C., Çinar M.E., Dubar J., Dubois S., Erga Z., Guillemain D., Sartoretto S., Thierry de Ville d’Avray L., Zuberer F., Chenuil A., Féral J.-P., (2014), with contributors : Açik Çinar S., Andral B., Aurelle D., Aysel V., Bakir K., Bellan G., Bellan-Santini D., Bouchoucha M., Celik C., Chatzigeorgiou G., Chatzinikolaou E., Chenesseau S., Dağli E., Dailianis T., Dimitriadis C., D’Iribarne C., Doğan A., Dounas C., Egea E., Elguerrabi W., Emery E., Evcen A., Faulwetter S., Gatti G., Gerovasileiou V., Güçver S.M., Issaris Y., Katağan T., Keklikoglou K., Kirkim F., Koçak F., Koutsoubas D., Marschal C., Önen M., Önen S., Öztürk B., Panayiotidis P., Pavloudi C., Pergent G., Pergent-Martini C., Poursanidis D., Ravel C., Reizopoulou S., Rocher C., Ruiton S., Sakher S., Salomidi M., Sarropoulou E., Selva M., Sini M., Sourbes L., Simboura N., Taşkin E., Vacelet J., Valavanis V., Vasileiadou A., Verlaque M. Protocols for monitoring of coralligenous habitats of mediterannean (Coralligenous based Indicators to Evaluate and Monitor the "good ecological status" of the MEDiterranean coastal waters) Protocoles de suivi du coralligène en méditerranée (Coralligenous
based Indicators to Evaluate and Monitor the "good ecological status" of the MEDiterranean coastal waters).

SAMPLING PROTOCOL FOR POPULATION GENETICS



(32 x samples from different colonies/thalli per species)

Method



  • 2 target species, widespread in all the Mediterranean se :
    - Myriapora truncata
    - Lithophyllum stictaeforme/cabiochiae
  • 2-3 fragments of Myriapora truncata from the apical extremity of the colonies,minimum size of 3 cm,
  • 3-4 fragments of Lithophyllum stictaeforme/cabiochiae, minimum size 3 cm



Expected sample



  • 32 samples of Myriapora truncata (2 profiles x 2 orientations x 8 replicates) collected at 28m deep. Potentially the same number of samples at 43m.
  • Typology of labelling samples of Myriapora truncata :
    [program]_[site?]_[date]_D[depth]_[species]_T[n°transect?]_S[n°segment]
    _R[n°replicate] _[doer]
  • 32 samples of Lithophyllum cabiochiae (2 profiles x 2 orientations x 8 replicates) collected at 28m deep. Potentially the same number of samples at 43m.
  • Typology of labelling samples of Lithophyllum cabiochiae :
    [program]_[site]_[date]_ D[depth]_[species]_T[n°transect]_S[n°segment]_R[n°replicate]_[doer]

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